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susanleemburg

@susanleemburg@neuromatch.social

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susanleemburg, to random
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Lessons learned I guess:

  1. "in duplo" means run 2 separate ELISA kits if your samples are precious,
  2. Just because it is a kit that costs half the yearly budget does not mean it will work (and you do not get to complain about it),
  3. Just redo an entire year worth of experiments basically.
susanleemburg, to Neuroscience
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O man... I thought that the OpenEphys ONIX system was just a new kind of headstage that works with most of their old hardware.

Does anyone have an ONIX? Is it worth it for just multichannel LFP or tetrode recordings?

susanleemburg, to random
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OMG but I hate Python.

I just want to run some spike detection code I made a while ago.
Instead of spikes I get a weird error. So now I need to update package 1, which requires updating package 2, 3, 4, 7, and 28, which in turn want a newer version of python (except package 9 which refuses to work now of course), so I also need to reinstall anaconda completely (fuck knows why the upgrade button never works)...
And of course none of that actually runs, so I need to figure out how to make things go in a docker container that is in turn wrapped in whatever the hell a singularity is?

susanleemburg, to random
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Achievement unlocked: I broke a neuropixels probe by sneezing on it #happyfriday

susanleemburg, to random
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We're having a bit of a problem with assembling our #neuropixels assembly rig (specifically with the docking holder for the Apollo implant for NP1):
When we try to heat-insert the
brass threads, the walls of the holder holes keep cracking and breaking.
We use a 350C soldering iron, but we have one with a pretty small tip.
Are our brass inserts still too cold? Are we inserting too fast? Any ideas what we might be doing wrong?

image/jpeg

susanleemburg, to random
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But susaaannnn we never get anything

susanleemburg, to random
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I like how spring is basically just all of nature going "hell yeah, let's party 🥳"

Photo of a tree stem with a small cluster of while apple flowers sprouting from it.

susanleemburg, to random
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Look, I just want to make filtfilt go faster by running it on my GPU. Matlab documentation says yes, matlab filtfilt says no.

How did I end up downloading a new matlab that now complains that my drivers are too old??

I'm a simple girl, I just want this phase-amplitude thing to take less than 3 days per recording. Just to make filtfilt go vroom 😖​

susanleemburg, to random
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In other weird and wonderful stuff in the cabinet-with-the-brain: a cheerful skull tattoo

susanleemburg, to random
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I guess I really need to go back and read the label. That's one weird looking section...

susanleemburg, to random
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Delightful thing of the day: this grafted little cherry tree is also making an attempt at apples

susanleemburg, to random
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We're having some trouble with isoflurane supply in CZ.
Is isoflurane being discontinued elsewhere in Europe too? Does anyone know?

susanleemburg, to random
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I'm not really able to explain to students why I think that using generative AI (e.g. chatGPT) for writing is bad. At least, not in a way that convinces anyone.

My argument is basically this: writing is thinking.
In order to write about research clearly, you need clear thinking. The act of writing a paper/report helps you think about it more clearly, because all those fuzzy gaps that your mind can just jump over show up very clearly on paper. So, writing is a way to figure out your own thoughts and not only a way to tell others about them.
I think that’s an essential part of doing science and something that you cannot and should not outsource to a machine.

I don't know how to express this in a way that makes sense to my students. Sigh.

susanleemburg, to random
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Me to students: comment your code! Be helpful to your future self!

Also me to me:

susanleemburg, to random
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Going to have my heart broken by the election results tonight 😭​, but I also have tickets for Castlefest 🧚​.

susanleemburg, to random
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Always fun to read still relevant laments in very old papers

susanleemburg, to random
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Does anyone know why Orchid and Teevra #neurons are called that?

susanleemburg, to neuro
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I've been wondering if we can make a regular (neuro) sciency movie night series at the department and have come to the sad conclusion that I do not have a long enough list of films that I like :(

So, what are your favorite (neuro) sciency films/documentaries?

#MovieNight #Neuro #SciEd

susanleemburg, to random
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So even in that latest c-fos paper, from the year 2023, the apparently gold standard antibody is still the discontinued santa cruz polyclonal? Are you kidding me?

It's of course possible that they saw a to-be-discontinued notice and thought "yikes, let's buy a few liters just in case", but the way this ghost Ab just keeps haunting the literature is really something else.

susanleemburg, to random
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Sometimes a #NeuroRat has no thoughts, just vibes

susanleemburg, to random
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OMG. At this stage of my life I should really know how to spell cannula at the first attempt.

susanleemburg, to neuro
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Gather 'round #neuro mastodons, we're going to read a paper and be a bit #interactive about it.

An Interpretation of Michelangelo's
Creation of Adam Based on Neuroanatomy, by Frank Lynn Meshberger, from JAMA, 10 October 1990.

doi:10.1001/jama.1990.03450140059034

#journalclub

susanleemburg, to random
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So my friend asked me to download a paper for her class, and it is so silly that I had to check that it wasn't published in the 'funny issue' (1 April or Christmas or something).

And I don't think so? The 10 October 1990 issue of JAMA doesn't seem to be a very silly one on purpose.

susanleemburg, to neuro
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Good afternoon to the coolest of #neuro people! What are your best/favorite #neuropixels headstages for freely moving rats?

susanleemburg, to random
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@elduvelle did you manage to find out anything more re: #mountainsort4 and #isosplit5?
I think I'm stuck at the same point as you (but then in win11).

Btw, I almost have klustakwik sorting for NLX figured out, I just need to test the export to phy. It's incredibly slow though.

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