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susanleemburg

@susanleemburg@neuromatch.social

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susanleemburg, to Neuroscience
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O man... I thought that the OpenEphys ONIX system was just a new kind of headstage that works with most of their old hardware.

Does anyone have an ONIX? Is it worth it for just multichannel LFP or tetrode recordings?
#neuroscience #neuromastodon

susanleemburg,
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I use rats and I was quite excited about the 2-wire cable, because I plan to do repeated 24-hour recordings in my rats
2 wires are (in theory at least) a lot more repairable than very thin 12-wire cables. The 12-pin omnetics plugs are extremely annoying for soldering.

susanleemburg,
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Also, I've yet to meet a rat who will never eat a cable

susanleemburg,
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Alternatively, a wireless system that can do ~2kHz sampling rate and doesn't have a pathetic little battery life of 2 hours would be great too.

susanleemburg, to random
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OMG but I hate Python.

I just want to run some spike detection code I made a while ago.
Instead of spikes I get a weird error. So now I need to update package 1, which requires updating package 2, 3, 4, 7, and 28, which in turn want a newer version of python (except package 9 which refuses to work now of course), so I also need to reinstall anaconda completely (fuck knows why the upgrade button never works)...
And of course none of that actually runs, so I need to figure out how to make things go in a docker container that is in turn wrapped in whatever the hell a singularity is?

susanleemburg,
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@jonny @neuralreckoning @elduvelle @briansimulator I'm much more at home in matlab than in python, so it's very likely that I'm a pretty big factor in whatever mess my python scripts end up being. But I'd like to not have to wonder if a year-old script still works, or if something has become not-quite compatible in the mean time. Particularly this morning when I had planned to to many other things instead. Sigh.

I guess my mess was the flipside of having the version-flexibility/specificity in python that you don't have in matlab (at least not in the same way).

I've never really gotten along with docker, so I mostly just run different anaconda environments for now. I refuse to figure out singularity out of spite, for the moment.

susanleemburg,
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@jonny @elduvelle I actually don't have a huge preference for matlab over python because of the software itself. It's really more based on my own skill.
The newer matlabs have a number of features that I absolutely despise (being able to edit the data directly without coding has to be some of the worst).

susanleemburg, to random
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Achievement unlocked: I broke a neuropixels probe by sneezing on it #happyfriday

susanleemburg, to random
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We're having a bit of a problem with assembling our #neuropixels assembly rig (specifically with the docking holder for the Apollo implant for NP1):
When we try to heat-insert the
brass threads, the walls of the holder holes keep cracking and breaking.
We use a 350C soldering iron, but we have one with a pretty small tip.
Are our brass inserts still too cold? Are we inserting too fast? Any ideas what we might be doing wrong?

image/jpeg

susanleemburg, to random
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But susaaannnn we never get anything

susanleemburg,
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@elduvelle I gave them all a special personal kibble, which they all took from me, and then dropped on the floor. Motherfluffers

susanleemburg, to random
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I like how spring is basically just all of nature going "hell yeah, let's party ๐Ÿฅณ"

Photo of a tree stem with a small cluster of while apple flowers sprouting from it.

susanleemburg,
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seriously, who needs branches? Just sprout flowers and leaves directly from the stem. #STEMenthusiast

susanleemburg,
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@jonny Right? It would be so cute

susanleemburg, to random
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Look, I just want to make filtfilt go faster by running it on my GPU. Matlab documentation says yes, matlab filtfilt says no.

How did I end up downloading a new matlab that now complains that my drivers are too old??

I'm a simple girl, I just want this phase-amplitude thing to take less than 3 days per recording. Just to make filtfilt go vroom ๐Ÿ˜–โ€‹

susanleemburg,
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So I guess I can add 72 days of calculation to this paper, or I can give up on having this one figure. Boo to me and my laptop.

susanleemburg,
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@jonny
Maybe? Reinstalling the latest driver that was already installed seems to have fixed the problem with matlab. I have no idea why this was the magic time.
I now have 3 different matlabs, which is honestly embarrassing but I can learn to live with it.

What I want to do is look at some phase amplitude coupling between a cortical and hippocampal LFP across a pretty wide range of frequencies (0.5 - 140Hz). I'm basing it on Tort et al., J Neurophysiol 2010 (https://github.com/tortlab/phase-amplitude-coupling)

For each frequency bin, there is a filfilt step, followed by a Hilbert transformation to get phase or amplitude, and then I calculate a modulation index for each phase/amplitude bin. I am not sure if the whole method is just very inefficient, or if it will just take a long time to calculate because of my data where I repeat this for a lot of epochs.

susanleemburg, to random
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In other weird and wonderful stuff in the cabinet-with-the-brain: a cheerful skull tattoo

susanleemburg,
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@jonny It's a display case in the main building of our faculty. I only recently discovered this anatomy one because I don't go there very often.

susanleemburg, to random
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Delightful thing of the day: this grafted little cherry tree is also making an attempt at apples

susanleemburg, to random
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I guess I really need to go back and read the label. That's one weird looking section...

susanleemburg,
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@jonny My guess is that the soft tissue has shrunk due to the fixation method and that there is some kind of tumor.
I can check at the next coffee break

susanleemburg, to random
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We're having some trouble with isoflurane supply in CZ.
Is isoflurane being discontinued elsewhere in Europe too? Does anyone know?

susanleemburg,
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I swear if this is going to be like "yep, no more meliodent cement anywhere, find something else", I'm going to throw a tiny tantrum.

And probably hamster a bunch of iso. Ugh.

susanleemburg,
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@jonny RIP our budget too... the new stuff is like 3x as expensive ๐Ÿ˜ญ

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