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susanleemburg

@susanleemburg@neuromatch.social

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susanleemburg, to random
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Lessons learned I guess:

  1. "in duplo" means run 2 separate ELISA kits if your samples are precious,
  2. Just because it is a kit that costs half the yearly budget does not mean it will work (and you do not get to complain about it),
  3. Just redo an entire year worth of experiments basically.
susanleemburg, to Neuroscience
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O man... I thought that the OpenEphys ONIX system was just a new kind of headstage that works with most of their old hardware.

Does anyone have an ONIX? Is it worth it for just multichannel LFP or tetrode recordings?

susanleemburg,
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I use rats and I was quite excited about the 2-wire cable, because I plan to do repeated 24-hour recordings in my rats
2 wires are (in theory at least) a lot more repairable than very thin 12-wire cables. The 12-pin omnetics plugs are extremely annoying for soldering.

susanleemburg,
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Also, I've yet to meet a rat who will never eat a cable

susanleemburg,
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Alternatively, a wireless system that can do ~2kHz sampling rate and doesn't have a pathetic little battery life of 2 hours would be great too.

susanleemburg, to random
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OMG but I hate Python.

I just want to run some spike detection code I made a while ago.
Instead of spikes I get a weird error. So now I need to update package 1, which requires updating package 2, 3, 4, 7, and 28, which in turn want a newer version of python (except package 9 which refuses to work now of course), so I also need to reinstall anaconda completely (fuck knows why the upgrade button never works)...
And of course none of that actually runs, so I need to figure out how to make things go in a docker container that is in turn wrapped in whatever the hell a singularity is?

susanleemburg,
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@jonny @neuralreckoning @elduvelle @briansimulator I'm much more at home in matlab than in python, so it's very likely that I'm a pretty big factor in whatever mess my python scripts end up being. But I'd like to not have to wonder if a year-old script still works, or if something has become not-quite compatible in the mean time. Particularly this morning when I had planned to to many other things instead. Sigh.

I guess my mess was the flipside of having the version-flexibility/specificity in python that you don't have in matlab (at least not in the same way).

I've never really gotten along with docker, so I mostly just run different anaconda environments for now. I refuse to figure out singularity out of spite, for the moment.

susanleemburg,
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@jonny @elduvelle I actually don't have a huge preference for matlab over python because of the software itself. It's really more based on my own skill.
The newer matlabs have a number of features that I absolutely despise (being able to edit the data directly without coding has to be some of the worst).

susanleemburg, to random
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Achievement unlocked: I broke a neuropixels probe by sneezing on it #happyfriday

susanleemburg, to random
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We're having a bit of a problem with assembling our assembly rig (specifically with the docking holder for the Apollo implant for NP1):
When we try to heat-insert the
brass threads, the walls of the holder holes keep cracking and breaking.
We use a 350C soldering iron, but we have one with a pretty small tip.
Are our brass inserts still too cold? Are we inserting too fast? Any ideas what we might be doing wrong?

image/jpeg

susanleemburg, to random
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But susaaannnn we never get anything

susanleemburg,
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@elduvelle I gave them all a special personal kibble, which they all took from me, and then dropped on the floor. Motherfluffers

susanleemburg, to random
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I like how spring is basically just all of nature going "hell yeah, let's party 🥳"

Photo of a tree stem with a small cluster of while apple flowers sprouting from it.

susanleemburg,
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seriously, who needs branches? Just sprout flowers and leaves directly from the stem. #STEMenthusiast

susanleemburg,
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@jonny Right? It would be so cute

susanleemburg, to random
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Look, I just want to make filtfilt go faster by running it on my GPU. Matlab documentation says yes, matlab filtfilt says no.

How did I end up downloading a new matlab that now complains that my drivers are too old??

I'm a simple girl, I just want this phase-amplitude thing to take less than 3 days per recording. Just to make filtfilt go vroom 😖​

susanleemburg,
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So I guess I can add 72 days of calculation to this paper, or I can give up on having this one figure. Boo to me and my laptop.

susanleemburg,
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@jonny
Maybe? Reinstalling the latest driver that was already installed seems to have fixed the problem with matlab. I have no idea why this was the magic time.
I now have 3 different matlabs, which is honestly embarrassing but I can learn to live with it.

What I want to do is look at some phase amplitude coupling between a cortical and hippocampal LFP across a pretty wide range of frequencies (0.5 - 140Hz). I'm basing it on Tort et al., J Neurophysiol 2010 (https://github.com/tortlab/phase-amplitude-coupling)

For each frequency bin, there is a filfilt step, followed by a Hilbert transformation to get phase or amplitude, and then I calculate a modulation index for each phase/amplitude bin. I am not sure if the whole method is just very inefficient, or if it will just take a long time to calculate because of my data where I repeat this for a lot of epochs.

susanleemburg, to random
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In other weird and wonderful stuff in the cabinet-with-the-brain: a cheerful skull tattoo

susanleemburg,
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@jonny It's a display case in the main building of our faculty. I only recently discovered this anatomy one because I don't go there very often.

susanleemburg, to random
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Delightful thing of the day: this grafted little cherry tree is also making an attempt at apples

susanleemburg, to random
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I guess I really need to go back and read the label. That's one weird looking section...

susanleemburg,
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@jonny My guess is that the soft tissue has shrunk due to the fixation method and that there is some kind of tumor.
I can check at the next coffee break

susanleemburg, to random
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We're having some trouble with isoflurane supply in CZ.
Is isoflurane being discontinued elsewhere in Europe too? Does anyone know?

susanleemburg,
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I swear if this is going to be like "yep, no more meliodent cement anywhere, find something else", I'm going to throw a tiny tantrum.

And probably hamster a bunch of iso. Ugh.

susanleemburg,
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@jonny RIP our budget too... the new stuff is like 3x as expensive 😭

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